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Original Research (Original Article) 


Preparation of fibrin glue from catfish blood and its application

Abo-Ghanema, I.I., Nemettallah, B.R., Naser, S.M., Mutkhatrid, R.A..

Abstract
Fibrin glue was prepared from catfish (Clarias lazera) blood by mixing the coagulation factors Fish fibrinogen, prothrombin extracted from fish blood and thromboplastin was prepared from fish muscles. The present study was conducted in fifteen adult male New Zealand white rabbits divided into three equal groups (n=5); Skin wounds group, Second-degree skin burn group and bleeding ear vein group to evaluate the healing potential of the prepared fibrin glue. The same animal was used as self-control for each treatment. Rabbits were anesthetized with Xylazine hydrochloride 5 mg/kg and Ketamine hydrochloride 35 mg/kg BW I/M. In the first group, dorsal skin incisions were created. In the second group, second-degree skin burns were induced. Wounds were treated with fibrin glue and/or saline as control. In the third group, hemostasis of the bleeding ear vessels was performed by applying a gauze pad soaked with fibrin glue in order to be compared with gauze of 0.9% normal saline. The biopsies were taken at 7th day in first group, on the 10th day in second group. Wound healing was monitored through macroscopic and histological examination. Treatment with fibrin glue increased the re-epithelialization and the regeneration of the epidermal granular layer in both skin incisions and skin burns. In the third group the mean bleeding time was reduced in treated-fibrin glue to 13.8 seconds compared with average 28 seconds in controls. It is suggested that fish fibrin glue could be used for wound and burn healing as well as for hemostasis purposes.

Key words: Catfish blood; Fibrin glue; Fibrinogen; Healing wound; Prothrombin


 
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How to Cite this Article
Pubmed Style

, I.I. , Nemettallah , B.R. , Naser , S.M. , Mutkhatrid , R.A. . Preparation of fibrin glue from catfish blood and its application. DJVS. 2019; 1(1): 1-4. doi:10.5455/DJVS.22432


Web Style

, I.I. , Nemettallah , B.R. , Naser , S.M. , Mutkhatrid , R.A. . Preparation of fibrin glue from catfish blood and its application. http://www.damanhourjvetsci.com/?mno=22432 [Access: August 26, 2019]. doi:10.5455/DJVS.22432


AMA (American Medical Association) Style

, I.I. , Nemettallah , B.R. , Naser , S.M. , Mutkhatrid , R.A. . Preparation of fibrin glue from catfish blood and its application. DJVS. 2019; 1(1): 1-4. doi:10.5455/DJVS.22432



Vancouver/ICMJE Style

, I.I. , Nemettallah , B.R. , Naser , S.M. , Mutkhatrid , R.A. . Preparation of fibrin glue from catfish blood and its application. DJVS. (2019), [cited August 26, 2019]; 1(1): 1-4. doi:10.5455/DJVS.22432



Harvard Style

, I.I., ., Nemettallah, ., B.R., ., Naser, ., S.M., ., Mutkhatrid, . & R.A., . (2019) Preparation of fibrin glue from catfish blood and its application. DJVS, 1 (1), 1-4. doi:10.5455/DJVS.22432



Turabian Style

, I.I., Nemettallah, B.R., Naser, S.M., Mutkhatrid, and R.A.. 2019. Preparation of fibrin glue from catfish blood and its application. Damanhour Journal of Veterinary Sciences, 1 (1), 1-4. doi:10.5455/DJVS.22432



Chicago Style

, I.I., Nemettallah, B.R., Naser, S.M., Mutkhatrid, and R.A.. "Preparation of fibrin glue from catfish blood and its application." Damanhour Journal of Veterinary Sciences 1 (2019), 1-4. doi:10.5455/DJVS.22432



MLA (The Modern Language Association) Style

, I.I., Nemettallah, B.R., Naser, S.M., Mutkhatrid, and R.A.. "Preparation of fibrin glue from catfish blood and its application." Damanhour Journal of Veterinary Sciences 1.1 (2019), 1-4. Print. doi:10.5455/DJVS.22432



APA (American Psychological Association) Style

, I.I., ., Nemettallah, ., B.R., ., Naser, ., S.M., ., Mutkhatrid, . & R.A., . (2019) Preparation of fibrin glue from catfish blood and its application. Damanhour Journal of Veterinary Sciences, 1 (1), 1-4. doi:10.5455/DJVS.22432