E-ISSN 2636-3003 | ISSN 2636-2996
 

Original Research (Original Article) 


Isolation, identification and antimicrobial susceptibility testing of recent E. coli serotypes from Japanese Quails reared in Sharkia Governorate, Egypt.

Walaa Fathy Saad Eldin Ibrahim.

Abstract
Quails are growing sources for animal-derived protein in Egypt. This study aimed at investigation of the prevalence of avian pathogenic E. coli (APEC) in quails in Sharkia Governorate, Egypt. Furthermore, serological identification of APEC strains was performed followed by screening of the expression of the virulence-associated genes and the antibiogram of the identified serotypes. The achieved results in the present study revealed that E. coli isolates were recovered at 28% of the examined quail samples. E. coli isolates were recovered from the different organs at variable percentages. Serological identification of the isolated E. coli demonstrated that five serotypes were identified, namely O2, O20, O35, O78 and O127. E. coli O 127 was the most predominant serotype (28.56%). E. coli O2 came second at 24.99%. The identified serotypes harbored virulence-associated genes including tsh, astA and iroN that facilitate microbial colonization, adhesion and dissemination into different tissue. Antimicrobial resistance profiling of the identified serotypes revealed their resistance to several antimicrobials commonly used in the poultry production in Egypt. On the same time, the identified E. coli serotypes showed marked sensitivity to gentamycin, neomycin, enrofloxacin and erythromycin indicating that these antimicrobials are promising candidates for the control of E. coli infection in quails.

Key words: E. coli; quails; antibiogram; virulence-associated genes


 
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How to Cite this Article
Pubmed Style

Walaa Fathy Saad Eldin Ibrahim. Isolation, identification and antimicrobial susceptibility testing of recent E. coli serotypes from Japanese Quails reared in Sharkia Governorate, Egypt.. DJVS. 2019; 1(2): 14-17. doi:10.5455/DJVS.38982


Web Style

Walaa Fathy Saad Eldin Ibrahim. Isolation, identification and antimicrobial susceptibility testing of recent E. coli serotypes from Japanese Quails reared in Sharkia Governorate, Egypt.. http://www.damanhourjvetsci.com/?mno=38982 [Access: September 22, 2019]. doi:10.5455/DJVS.38982


AMA (American Medical Association) Style

Walaa Fathy Saad Eldin Ibrahim. Isolation, identification and antimicrobial susceptibility testing of recent E. coli serotypes from Japanese Quails reared in Sharkia Governorate, Egypt.. DJVS. 2019; 1(2): 14-17. doi:10.5455/DJVS.38982



Vancouver/ICMJE Style

Walaa Fathy Saad Eldin Ibrahim. Isolation, identification and antimicrobial susceptibility testing of recent E. coli serotypes from Japanese Quails reared in Sharkia Governorate, Egypt.. DJVS. (2019), [cited September 22, 2019]; 1(2): 14-17. doi:10.5455/DJVS.38982



Harvard Style

Walaa Fathy Saad Eldin Ibrahim (2019) Isolation, identification and antimicrobial susceptibility testing of recent E. coli serotypes from Japanese Quails reared in Sharkia Governorate, Egypt.. DJVS, 1 (2), 14-17. doi:10.5455/DJVS.38982



Turabian Style

Walaa Fathy Saad Eldin Ibrahim. 2019. Isolation, identification and antimicrobial susceptibility testing of recent E. coli serotypes from Japanese Quails reared in Sharkia Governorate, Egypt.. Damanhour Journal of Veterinary Sciences, 1 (2), 14-17. doi:10.5455/DJVS.38982



Chicago Style

Walaa Fathy Saad Eldin Ibrahim. "Isolation, identification and antimicrobial susceptibility testing of recent E. coli serotypes from Japanese Quails reared in Sharkia Governorate, Egypt.." Damanhour Journal of Veterinary Sciences 1 (2019), 14-17. doi:10.5455/DJVS.38982



MLA (The Modern Language Association) Style

Walaa Fathy Saad Eldin Ibrahim. "Isolation, identification and antimicrobial susceptibility testing of recent E. coli serotypes from Japanese Quails reared in Sharkia Governorate, Egypt.." Damanhour Journal of Veterinary Sciences 1.2 (2019), 14-17. Print. doi:10.5455/DJVS.38982



APA (American Psychological Association) Style

Walaa Fathy Saad Eldin Ibrahim (2019) Isolation, identification and antimicrobial susceptibility testing of recent E. coli serotypes from Japanese Quails reared in Sharkia Governorate, Egypt.. Damanhour Journal of Veterinary Sciences, 1 (2), 14-17. doi:10.5455/DJVS.38982